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The formation of fusion genes results in overexpression of TFE3 fusion protein, which present with strong nuclear expression in immunohistochemistry (IHC).9 Moderately to strongly positive nuclear immunoreactivity of TFE3 is an important clue to the diagnosis of Xp11.2 tRCCs, while false-negativity and positivity due to different technical methods reduce its reliability.21 Fluorescence in situ hybridization (FISH) assay is an effective ...
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