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Establishment of human NB cell line with upregulated expression of SIRT4 SIRT4 gene expression in SIRT4-expressing cell lines was stably upregulated compared to human NB cell lines transfected with the empty vector (SH-SY5Y, SK-N-BE, and IMR-32), as shown by RT-qPCR, Western blotting, and immunofluorescence, and the gene expression of SIRT4 in SIRT4-over-express cell lines was stably upregulated (...
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