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41467 2021 25604 Fig1 HTML.
Immunoprecipitated chromatin was then incubated with washed beads for 1 h rotating at 4 °C and washed twice sequentially with wash buffer 1 (20 mM Tris-HCl pH 7.5, 2 mM EDTA, 150 mM NaCl, 1% SDS, 1% Triton-X100), wash buffer 2 (20 mM Tris-HCl pH 7.5, 2 mM EDTA, 500 mM NaCl, 0.1% SDS, 1% Triton-X100), wash buffer 3 (10 mM Tris-HCl pH 7.5, 1 mM EDTA, 250 mM LiCl, 1% NP-40, 1% Na-deoxycholate) and 10 mM Tris-HCl pH 8.0, using a cold magnet (Invitrogen).
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